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Literature summary extracted from

  • Steinbach, A.K.; Fraas, S.; Harder, J.; Tabbert, A.; Brinkmann, H.; Meyer, A.; Ermler, U.; Kroneck, P.M.
    Cyclohexane-1,2-dione hydrolase from denitrifying Azoarcus sp. strain 22Lin, a novel member of the thiamine diphosphate enzyme family (2011), J. Bacteriol., 193, 6760-6769.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.7.1.11 Cyclohexane-1,2-dione substrate inhibition above 0.1 mM Azoarcus sp.
3.7.1.11 additional information activity decreases significantly at this high ionic strength Azoarcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.7.1.11 Mg2+ 1.0 Mg2+ molecule per enzyme monomer Azoarcus sp.
3.7.1.11 additional information activity decreases significantly at this high ionic strength Azoarcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.11 59000
-
 2 * 59000, SDS-PAGE, 2 * 64500, about, sequence calculation Azoarcus sp.
3.7.1.11 64500
-
 2 * 59000, SDS-PAGE, 2 * 64500, about, sequence calculation Azoarcus sp.
3.7.1.11 105000
-
 gel filtration Azoarcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.7.1.11 cyclohexane-1,2-dione + H2O Azoarcus sp. degradation of cyclohexane-1,2-dione to 6-oxohexanoate comprises the cleavage of a COC bond adjacent to a carbonyl group. In the subsequent NAD-dependent reaction, 6-oxohexanoate is oxidized to adipate 6-oxohexanoate + ? further convertion to adipate using NAD+ as electron acceptor ?
3.7.1.11 cyclohexane-1,2-dione + H2O Azoarcus sp. 22Lin degradation of cyclohexane-1,2-dione to 6-oxohexanoate comprises the cleavage of a COC bond adjacent to a carbonyl group. In the subsequent NAD-dependent reaction, 6-oxohexanoate is oxidized to adipate 6-oxohexanoate + ? further convertion to adipate using NAD+ as electron acceptor ?

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.11 Azoarcus sp. P0CH62
-
-
3.7.1.11 Azoarcus sp. 22Lin P0CH62
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.11 native CDH 6.6fold by two different steps of anion exchange chromatography, followed by gel filtration Azoarcus sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
3.7.1.11 cyclohexane-1,2-dione + H2O = 6-oxohexanoate catalytic reaction mechanism, overview Azoarcus sp.
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7.1.11 0.677
-
 purified enzyme, pH 8.0, 37°C Azoarcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.11 cyclohexane-1,2-dione + H2O
-
 Azoarcus sp. 6-oxohexanoate + ? further convertion to adipate using NAD+ as electron acceptor ?
3.7.1.11 cyclohexane-1,2-dione + H2O degradation of cyclohexane-1,2-dione to 6-oxohexanoate comprises the cleavage of a COC bond adjacent to a carbonyl group. In the subsequent NAD-dependent reaction, 6-oxohexanoate is oxidized to adipate Azoarcus sp. 6-oxohexanoate + ? further convertion to adipate using NAD+ as electron acceptor ?
3.7.1.11 cyclohexane-1,2-dione + H2O
-
 Azoarcus sp. 22Lin 6-oxohexanoate + ? further convertion to adipate using NAD+ as electron acceptor ?
3.7.1.11 cyclohexane-1,2-dione + H2O degradation of cyclohexane-1,2-dione to 6-oxohexanoate comprises the cleavage of a COC bond adjacent to a carbonyl group. In the subsequent NAD-dependent reaction, 6-oxohexanoate is oxidized to adipate Azoarcus sp. 22Lin 6-oxohexanoate + ? further convertion to adipate using NAD+ as electron acceptor ?

Subunits

EC Number Subunits Comment Organism
3.7.1.11 dimer 2 * 59000, SDS-PAGE, 2 * 64500, about, sequence calculation Azoarcus sp.

Synonyms

EC Number Synonyms Comment Organism
3.7.1.11 Cdh
-
 Azoarcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.7.1.11 37
-
 assay at Azoarcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.11 8
-
 assay at Azoarcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
3.7.1.11 FAD 1.0 FAD molecule per enzyme monomer, 2fold Rossmann fold for FAD binding at the C-terminal end Azoarcus sp.
3.7.1.11 thiamine diphosphate dependent on, 0.8 ThDP per enzyme monomer, ThDP binding motif, overview Azoarcus sp.

General Information

EC Number General Information Comment Organism
3.7.1.11 evolution the ring-cleaving cyclohexane-1,2-dione hydrolase is a member of the thiamine diphosphate enzyme family Azoarcus sp.
3.7.1.11 physiological function CDH catalyses a key step of an anaerobic degradation pathway for alicyclic alcohols by converting cyclohexane-1,2-dione to 6-oxohexanoate and further to adipate using NAD+ as electron acceptor Azoarcus sp.